Graduation Year

2011

Date of Thesis Acceptance

Fall 5-9-2011

Major Department or Program

Biology

Advisor(s)

Leena Knight

Abstract

Parkinson’s disease (PD) is most commonly associated with dopaminergic neuronal loss in the substantia nigra (SN), but recent evidence suggests that concomitant loss of noradrenergic neurons in the locus coeruleus (LC) also heavily contributes to PD pathology and symptomology. The primary goal of this study was to determine whether focal injection of 6-hydroxydopamine (6-OHDA), a neurotoxin to which noradrenergic cells are particularly susceptible, induces sufficient LC damage in mice to be comparable to the Parkinsonian brain (~ 50% loss). Coupling this LC model with injections of MPTP, a neurotoxin that preferentially targets and destroys dopaminergic neurons, allowed us to simulate PD disease progression and to observe the neuroprotective effects of the LC on the SN. In general, this report provides preliminary data in support of utilizing combined 6-OHDA/MPTP injections to model PD pathology in mice and to inform future therapeutic strategies.

Page Count

33

Subject Headings

Parkinson’s disease -- Prevention & control, Dopaminergic neurons, Substantia nigra, Locus coeruleus, Injections, Neurotoxic agents -- Toxicology, Oxidopamine -- 6-hydroxydopamine, Brain -- Diseases, Nervous system - Damage, Enzymes -- Synthesis, Animals -- Testing, Parkinsonian Disorders -- Therapy, Whitman College 2011 -- Dissertation collection -- Biology Department

Permanent URL

http://hdl.handle.net/10349/1011

Document Type

Public Accessible Thesis

Terms of Use

If you have questions about permitted uses of this content, please contact the ARMINDA administrator

Included in

Biology Commons

Share

COinS
 

Rights Statement

Rights Statement

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).