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Carbohydrates are vital to countless biological processes, but their diversity and structural and synthetic complexity necessitate the development of new investigative methods to rapidly examine a large variety of carbohydrate interactions. The microarray platform allows such high-throughput investigations, but is limited by the inconsistency of microarray fabrication, particularly the lack of control of carbohydrate density in printed spots. Addition of the anti-evaporative agents glycerol and dimethylformamide (DMF) failed to normalize printed carbohydrate density. Imaging the arrays with a combination of time-of-flight mass spectrometry (ToF-SIMS) and surface plasmon resonance (SPR) techniques effectively visualized both the chemical and biological activity of microarray surfaces, representing a new methodology for further microarray investigation.
Carbohydrates -- Chemistry, Carbohydrates, Glycomics -- Structure, Glycomics -- Data processing, Time-of=flight mass spectrometry -- Time-of-Flight-Secondary Ion Mass Spectrometry, Surface plasmon resonance -- Technological innovations -- Imaging, Surface chemistry, Microarray Analysis, Whitman College -- Dissertation collection 2011 -- Biology Department
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