Title

Effects of the PIRL9 Gene in Arabidopsis

Presenter

Ben Sheppard

Abstract

Arabidopsis thaliana is a small flowering plant native to Eurasia that is used by geneticists to study plants. Geneticists often study groups of genes or “gene families.” The PIRL family is made up of nine related genes which all share certain characteristics that are unusual in plants. Functional genomics attempts to determine the function of genes, and one approach is to create an over-expression construct. An over-expression construct is a piece of DNA inserted into the plant’s genome to create many extra copies of a gene product, in this case, protein. This approach has proved successful in the study of the the PIRL9 gene. In Arabdiopis extra PIRL9 protein creates a stunting or dwarf-like effect in the plants which contain the construct. This effect has been quantitatively measured in both the roots and leaves of experimental plants, indicating an important role for PIRL9 in plant development.

Faculty Sponsor

Dan Vernon

Sponsor Department/Programs

Biology

Tracks

Plant Life

Location

Science 159

Presentation Type

Oral Presentation

Research Funding Source or OCS Program

This research was funded by the Perry Summer Research Grant and the Abshire Student

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Apr 19th, 2:30 PM Apr 19th, 2:45 PM

Effects of the PIRL9 Gene in Arabidopsis

Science 159

Arabidopsis thaliana is a small flowering plant native to Eurasia that is used by geneticists to study plants. Geneticists often study groups of genes or “gene families.” The PIRL family is made up of nine related genes which all share certain characteristics that are unusual in plants. Functional genomics attempts to determine the function of genes, and one approach is to create an over-expression construct. An over-expression construct is a piece of DNA inserted into the plant’s genome to create many extra copies of a gene product, in this case, protein. This approach has proved successful in the study of the the PIRL9 gene. In Arabdiopis extra PIRL9 protein creates a stunting or dwarf-like effect in the plants which contain the construct. This effect has been quantitatively measured in both the roots and leaves of experimental plants, indicating an important role for PIRL9 in plant development.