Title

Sexual Dimorphism in Dopaminergic VTA Neurons and Their Presynaptic Partners

Presenter

Kathryn Beebe

Abstract

A brain region called the ventral tegmental area (VTA) is a group of predominantly dopamine-producing (DA) neurons involved in reward circuitry and addiction and is implicated in diseases such as Parkinson’s and schizophrenia. Current studies suggest differences in inputs (from the dorsal raphe nucleus) onto DA neurons in the VTA between male and female adult mice. It is not yet clear whether these differences are due to differences between sexes in the number of DA neurons in the VTA or in the effectiveness of viral injection techniques. To answer this question, we replicated methods of viral-labeling of inputs to the VTA (using modified A AV virus and mCherry rabies virus) and methods of immunohistochemistry (mCherry, GFP, and TH staining). Preliminary results suggest that male and female mice show equal numbers of DA neurons across the VTA and that the viral injection worked as expected and equally in both sexes of mice.

Faculty Sponsor

Thomas Knight

Sponsor Department/Programs

Biology

Tracks

Poster Session

Terms of Use

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Location

Cordiner Hall

Presentation Type

Poster

Research Funding Source or OCS Program

Whitman Internship Grant

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Apr 19th, 1:00 PM Apr 19th, 2:00 PM

Sexual Dimorphism in Dopaminergic VTA Neurons and Their Presynaptic Partners

Cordiner Hall

A brain region called the ventral tegmental area (VTA) is a group of predominantly dopamine-producing (DA) neurons involved in reward circuitry and addiction and is implicated in diseases such as Parkinson’s and schizophrenia. Current studies suggest differences in inputs (from the dorsal raphe nucleus) onto DA neurons in the VTA between male and female adult mice. It is not yet clear whether these differences are due to differences between sexes in the number of DA neurons in the VTA or in the effectiveness of viral injection techniques. To answer this question, we replicated methods of viral-labeling of inputs to the VTA (using modified A AV virus and mCherry rabies virus) and methods of immunohistochemistry (mCherry, GFP, and TH staining). Preliminary results suggest that male and female mice show equal numbers of DA neurons across the VTA and that the viral injection worked as expected and equally in both sexes of mice.

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