Title

Immunostaining for p300 and CBP

Abstract

My presentation focuses on research I conducted using immunohistochemistry to identify proteins in insulin-stimulated pathways. My research contributed to our lab's ability to analyze p300 and CBP proteins for their use of acetylation. Phosphorylation has long been seen as the primary driving force of downstream signaling. Our lab examined the importance and underestimation of acetylation. Initially, the lab used Western Blotting to look for protein presence, but the results we obtained were faint and difficult to analyze. I was assigned the task of troubleshooting a protocol for the staining of these two proteins. I sliced tissue, plated it, stained it and calculated the changes between knockout- and wild-type tissue. I was ultimately successful in identifying p300 but statistically unsuccessful in identifying CBP due to a technical error.

Faculty Sponsor

Paul Yancey

Tracks

Micro-science

Terms of Use

ARMINDA Terms of Use

Location

Science 159

Presentation Type

Oral Presentation

Research Funding Source or OCS Program

Whitman Internship Grant

This document is currently not available here.

Share

COinS
 
Apr 11th, 2:00 PM Apr 11th, 2:15 PM

Immunostaining for p300 and CBP

Science 159

My presentation focuses on research I conducted using immunohistochemistry to identify proteins in insulin-stimulated pathways. My research contributed to our lab's ability to analyze p300 and CBP proteins for their use of acetylation. Phosphorylation has long been seen as the primary driving force of downstream signaling. Our lab examined the importance and underestimation of acetylation. Initially, the lab used Western Blotting to look for protein presence, but the results we obtained were faint and difficult to analyze. I was assigned the task of troubleshooting a protocol for the staining of these two proteins. I sliced tissue, plated it, stained it and calculated the changes between knockout- and wild-type tissue. I was ultimately successful in identifying p300 but statistically unsuccessful in identifying CBP due to a technical error.

Rights Statement

Rights Statement

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).