Title

How Does Overactive EGF Signaling Affect C.elegans Lipidome

Abstract

Epidermal Growth Factor signaling is critical to animal development; it is also a hallmark of many cancers. Recent research in the nematode Caenorhabditis elegans has shown a role for EGF signaling in the control of lipid synthesis, via the lipid transcription factor SBP-1. This role led us to the question: How do different levels of EGF signaling affect the spectrum of lipids produced? I used mass spectrometry to assay the entire C. elegans lipidome under normal and overactive EGF signaling. To do so, I carried out a whole fat extraction, separated the lipids by TripleTOF, then analyzed the results for distinct lipid species and their relative quantities. I found that polyunsaturated lipids are widely trending up vs. normal, while mono-/saturated lipid production is trending down. Other emerging results suggest that lipid trends in overactive EGF signaling and sbp-1 mutants aren't as tightly correlated as we had hypothesized.

Faculty Sponsor

Matthew Crook

Tracks

Micro-science

Terms of Use

ARMINDA Terms of Use

Location

Science 159

Presentation Type

Oral Presentation

Research Funding Source or OCS Program

Robert Norton Fund

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Apr 11th, 2:45 PM Apr 11th, 3:00 PM

How Does Overactive EGF Signaling Affect C.elegans Lipidome

Science 159

Epidermal Growth Factor signaling is critical to animal development; it is also a hallmark of many cancers. Recent research in the nematode Caenorhabditis elegans has shown a role for EGF signaling in the control of lipid synthesis, via the lipid transcription factor SBP-1. This role led us to the question: How do different levels of EGF signaling affect the spectrum of lipids produced? I used mass spectrometry to assay the entire C. elegans lipidome under normal and overactive EGF signaling. To do so, I carried out a whole fat extraction, separated the lipids by TripleTOF, then analyzed the results for distinct lipid species and their relative quantities. I found that polyunsaturated lipids are widely trending up vs. normal, while mono-/saturated lipid production is trending down. Other emerging results suggest that lipid trends in overactive EGF signaling and sbp-1 mutants aren't as tightly correlated as we had hypothesized.

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