Title

Analysis of Candidate Genes for Gain and Loss of Anthocyanin Pigmentation in Mimulus cupreus

Abstract

What genetic mechanisms have allowed life to evolve such great diversity? One answer to this question is gene duplication, which allows new gene copies to take on novel functions. In the monkeyflower genus Mimulus, duplication of an anthocyanin-regulating Myb transcription factor gene has been associated with independent gains of anthocyanin pigmentation in multiple species. My presentation explores which Myb copy is responsible for gain and subsequent loss of anthocyanin pigmentation in Mimulus cupreus. Through transcriptome analysis and PCR, I identified and analyzed candidate Myb copies. One promising candidate was found to have a previously unknown splice variant in the pigmented morph and a large deletion in the unpigmented morph. Future research will include gene silencing and rescue to definitively test the contributions of this duplicated gene to flower color diversity, both within and between species.

Faculty Sponsor

Arielle Cooley

Tracks

Plant Wars

Terms of Use

ARMINDA Terms of Use

Location

Science 100

Presentation Type

Oral Presentation

This document is currently not available here.

Share

COinS
 
Apr 11th, 4:15 PM Apr 11th, 4:30 PM

Analysis of Candidate Genes for Gain and Loss of Anthocyanin Pigmentation in Mimulus cupreus

Science 100

What genetic mechanisms have allowed life to evolve such great diversity? One answer to this question is gene duplication, which allows new gene copies to take on novel functions. In the monkeyflower genus Mimulus, duplication of an anthocyanin-regulating Myb transcription factor gene has been associated with independent gains of anthocyanin pigmentation in multiple species. My presentation explores which Myb copy is responsible for gain and subsequent loss of anthocyanin pigmentation in Mimulus cupreus. Through transcriptome analysis and PCR, I identified and analyzed candidate Myb copies. One promising candidate was found to have a previously unknown splice variant in the pigmented morph and a large deletion in the unpigmented morph. Future research will include gene silencing and rescue to definitively test the contributions of this duplicated gene to flower color diversity, both within and between species.

Rights Statement

Rights Statement

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).