Title

How the PIRL6 Gene Effects Female Gametophyte Development in Arabidopsis Thaliana

Abstract

The female gametophyte is a vital structure for angiosperm reproduction that produces the egg cell, central cell and, following fertilization, the seed. Female gametophyte development in the Arabidopsis consists of eight morphologically distinct steps that are controlled by a series of genes. The PIRL genes are a family of nine genes that code for proteins similar to animal Ras-group leucine-rich repeat proteins, which are known to take part in developmental cell signaling. Application of the gene silencing method known as RNA interference was applied to the PIRL6 gene in Arabidopsis thaliana plants to investigate its possible involvement in the development of the female gametophyte. I report on the isolation of numerous PIRL6-silenced plants and show that decreased PIRL6 expression affects early female gametophyte development, resulting in an increased amount of aborted ovules and stunted developmental growth.

Faculty Sponsor

Dan Vernon, Nancy Forsthoefel

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poster

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Location

Cordiner Hall

Presentation Type

Poster

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How the PIRL6 Gene Effects Female Gametophyte Development in Arabidopsis Thaliana

Cordiner Hall

The female gametophyte is a vital structure for angiosperm reproduction that produces the egg cell, central cell and, following fertilization, the seed. Female gametophyte development in the Arabidopsis consists of eight morphologically distinct steps that are controlled by a series of genes. The PIRL genes are a family of nine genes that code for proteins similar to animal Ras-group leucine-rich repeat proteins, which are known to take part in developmental cell signaling. Application of the gene silencing method known as RNA interference was applied to the PIRL6 gene in Arabidopsis thaliana plants to investigate its possible involvement in the development of the female gametophyte. I report on the isolation of numerous PIRL6-silenced plants and show that decreased PIRL6 expression affects early female gametophyte development, resulting in an increased amount of aborted ovules and stunted developmental growth.

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