Title

Developing a Plant Hormone Biosensor that Utilizes Tandem Fluorescent Proteins

Abstract

Auxin is a plant hormone that is essential for plant growth and development and is found in all plant species. It is important to further understand how auxin concentrations vary within different plant tissues because the information gives insight to how this hormone is transported, perceived and metabolized. One method for detecting auxin is by biosensor, which enables the hormone to be sensed by harnessing the natural cellular machinery present in plant cells. We have begun pilot studies to develop a novel auxin biosensor that will emit fluorescence in the presence of auxin. We have designed two initial test biosensors that use green and red fluorescent proteins fused together such that exciting one of the proteins excites the other. Using a yeast cell system, our pilot experiments are focused on testing the functionality of these two initial test biosensors.

Faculty Sponsor

Britney Moss

Tracks

poster

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Location

Cordiner Hall

Presentation Type

Poster

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Developing a Plant Hormone Biosensor that Utilizes Tandem Fluorescent Proteins

Cordiner Hall

Auxin is a plant hormone that is essential for plant growth and development and is found in all plant species. It is important to further understand how auxin concentrations vary within different plant tissues because the information gives insight to how this hormone is transported, perceived and metabolized. One method for detecting auxin is by biosensor, which enables the hormone to be sensed by harnessing the natural cellular machinery present in plant cells. We have begun pilot studies to develop a novel auxin biosensor that will emit fluorescence in the presence of auxin. We have designed two initial test biosensors that use green and red fluorescent proteins fused together such that exciting one of the proteins excites the other. Using a yeast cell system, our pilot experiments are focused on testing the functionality of these two initial test biosensors.

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