Title

Surrogate Multidrug Resistance Evolution: Bacillus anthracis Sterne and Yersinia pestis A1122, KIM6, & KIM10,

Abstract

The dramatic increase in bacterial pathogens acquiring multidrug resistance poses a threat to global health and security, especially when they are intentionally used as biological warfare agents. To mitigate this risk, non-pathogenic MDR surrogates were generated to understand the genomic evolution of MDR. Surrogates permit safe testing of novel candidate drugs without the need for costly and highly regulated facilities. To generate MDR surrogates, B. anthracis Sterne and Y. pestis were evolved to be resistant to various antibiotics. Bacteria were subjected to selective pressures on plates containing three times the minimum inhibitory concentration of specific antibiotics. Resistant mutants were isolated and used for subsequent selection rounds with other antibiotics. The MDR surrogate isolates will be sequenced and compared to their parental strains to verify the presence of genes conferring resistance. The MDR strains will form a validated panel that can be used in reference laboratories to test new therapeutics.

Faculty Sponsor

Matt Craig

Tracks

poster

Terms of Use

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Location

Cordiner Hall

Presentation Type

Poster

Research Funding Source or OCS Program

Los Alamos National Laboratory, Bioscience Division

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Surrogate Multidrug Resistance Evolution: Bacillus anthracis Sterne and Yersinia pestis A1122, KIM6, & KIM10,

Cordiner Hall

The dramatic increase in bacterial pathogens acquiring multidrug resistance poses a threat to global health and security, especially when they are intentionally used as biological warfare agents. To mitigate this risk, non-pathogenic MDR surrogates were generated to understand the genomic evolution of MDR. Surrogates permit safe testing of novel candidate drugs without the need for costly and highly regulated facilities. To generate MDR surrogates, B. anthracis Sterne and Y. pestis were evolved to be resistant to various antibiotics. Bacteria were subjected to selective pressures on plates containing three times the minimum inhibitory concentration of specific antibiotics. Resistant mutants were isolated and used for subsequent selection rounds with other antibiotics. The MDR surrogate isolates will be sequenced and compared to their parental strains to verify the presence of genes conferring resistance. The MDR strains will form a validated panel that can be used in reference laboratories to test new therapeutics.

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