Title

EsaI Inhibition: Suppressing Virulence of Pantoea stewartii Through Quorum Sensing

Abstract

My research involved the bacteria Pantoea stewartii, which uses an enzyme called EsaI in order to produce signaling molecules. The bacteria are able to use these signaling molecules to measure the population density of neighboring bacteria. Once the population of bacteria reaches a particular threshold, it can target the expression of specific genes that promote the spread of infection. The primary goal of my research is to study how inhibiting EsaI may prevent Pantoea stewartii from being able to produce signaling molecules, in turn hindering the bacteria from expressing virulence genes. Since EsaI produces signaling molecules using a highly unstable native substrate, I helped synthesize alternative substrates that would enable the lab to conduct further studies on EsaI inhibition. The compatibility between EsaI and each synthesized substrate was compared to that of the native substrate in order to identify which synthesized substrates are viable alternatives to the native substrate.

Faculty Sponsor

Britney Moss

Tracks

poster

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Location

Cordiner Hall

Presentation Type

Poster

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EsaI Inhibition: Suppressing Virulence of Pantoea stewartii Through Quorum Sensing

Cordiner Hall

My research involved the bacteria Pantoea stewartii, which uses an enzyme called EsaI in order to produce signaling molecules. The bacteria are able to use these signaling molecules to measure the population density of neighboring bacteria. Once the population of bacteria reaches a particular threshold, it can target the expression of specific genes that promote the spread of infection. The primary goal of my research is to study how inhibiting EsaI may prevent Pantoea stewartii from being able to produce signaling molecules, in turn hindering the bacteria from expressing virulence genes. Since EsaI produces signaling molecules using a highly unstable native substrate, I helped synthesize alternative substrates that would enable the lab to conduct further studies on EsaI inhibition. The compatibility between EsaI and each synthesized substrate was compared to that of the native substrate in order to identify which synthesized substrates are viable alternatives to the native substrate.

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