Title

Utilization of Caenorhabditis elegans as a Model to Study Infection by Bartonella bacilliformis

Abstract

I studied the use of Caenorhabditis elegans as a model organism for the infection by Bartonella bacilliformis. Bartonella bacilliformis is an infectious bacteria that affects South American populations and especially Peru. It causes Carrion´s disease or Oroya fever in an acute phase and can also cause the chronic phase of Verruga peruana, or Peruvian warts. In my study, I explored methods of feeding B. bacilliformis to C. elegans to induce a phenotype in C. elegans. The best media to induce a disease phenotype in C. elegans was discovered after many trials: the Heart Infusion Broth with sheep blood (HIB-B) media inoculated with B. bacilliformis. C. elegans successfully infected with B. bacilliformis developed a Deformed Anal Region (DAR), a bump on the tail. I showed that C. elegans was a potential model organism to study mechanisms of B. bacilliformis infection as well as future vaccines.

Faculty Sponsor

Matthew Crook

Tracks

poster

Terms of Use

If you have questions about permitted uses of this content, please contact the ARMINDA administrator

Location

Cordiner Hall

Presentation Type

Poster

This document is currently not available here.

Share

COinS
 
Apr 11th, 1:00 PM Apr 11th, 2:00 PM

Utilization of Caenorhabditis elegans as a Model to Study Infection by Bartonella bacilliformis

Cordiner Hall

I studied the use of Caenorhabditis elegans as a model organism for the infection by Bartonella bacilliformis. Bartonella bacilliformis is an infectious bacteria that affects South American populations and especially Peru. It causes Carrion´s disease or Oroya fever in an acute phase and can also cause the chronic phase of Verruga peruana, or Peruvian warts. In my study, I explored methods of feeding B. bacilliformis to C. elegans to induce a phenotype in C. elegans. The best media to induce a disease phenotype in C. elegans was discovered after many trials: the Heart Infusion Broth with sheep blood (HIB-B) media inoculated with B. bacilliformis. C. elegans successfully infected with B. bacilliformis developed a Deformed Anal Region (DAR), a bump on the tail. I showed that C. elegans was a potential model organism to study mechanisms of B. bacilliformis infection as well as future vaccines.

Rights Statement

Rights Statement

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).