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Growth, Expression, and Characterization of Two Putative Hydroquinone Dioxygenases, MhqO and MnpC
April 12, 2016
Department or Program
PcpA is an enzyme involved in the degradation of the pollutant pentachlorophenol. It is a hydroquinone ring- cleaving dioxygenase (HQDO), and it oxidatively cleaves 2,6-dichlorohydroquinone. In order to better characterize and understand the specificity of PcpA, homologs of PcpA were studied. The homologs, selected based on sequence similarity, were procured and cloned into appropriate vectors, if necessary. Of the homologs grown and expressed, MnpC and MhqO currently have the most promise in demonstrating activity with hydroquinones. Recent experiments show 2-hydroxylhydroquinone as a possible substrate for MnpC, and 2-methylhydroquinone as a possible substrate for MhqO. Kinetic studies of these enzymes will help to elucidate more information about their binding affinities for their respective substrates. This information, coupled with ongoing and completed research, may help to further clarify the specificity of HQDOs; in the future, this information may be relevant to environmental remediation of chlorinated aromatic>hydrocarbons.