Graduation Year


Date of Thesis Acceptance

Fall 5-11-2011

Major Department or Program



Douglas Juers


Carbohydrates are vital to countless biological processes, but their diversity and structural and synthetic complexity necessitate the development of new investigative methods to rapidly examine a large variety of carbohydrate interactions. The microarray platform allows such high-throughput investigations, but is limited by the inconsistency of microarray fabrication, particularly the lack of control of carbohydrate density in printed spots. Addition of the anti-evaporative agents glycerol and dimethylformamide (DMF) failed to normalize printed carbohydrate density. Imaging the arrays with a combination of time-of-flight mass spectrometry (ToF-SIMS) and surface plasmon resonance (SPR) techniques effectively visualized both the chemical and biological activity of microarray surfaces, representing a new methodology for further microarray investigation.

Page Count


Subject Headings

Carbohydrates -- Chemistry, Carbohydrates, Glycomics -- Structure, Glycomics -- Data processing, Time-of=flight mass spectrometry -- Time-of-Flight-Secondary Ion Mass Spectrometry, Surface plasmon resonance -- Technological innovations -- Imaging, Surface chemistry, Microarray Analysis, Whitman College -- Dissertation collection 2011 -- Biology Department

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Public Accessible Thesis

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Biology Commons



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